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The primary advantages of these units are their ability to get reproducible elution volume and peak region, no matter cell stage viscosity or column blockages (Inside the strain limit from the HPLC pump).• Molecules with modest variations in absorption wavelengths may be detected effectively due to their dissimilarities in separation time. I.e.,
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Fig. 3 shows an instance through which the yellow ingredient has a solid affinity Using the mobile phase and moves rapidly via the column, while the pink component has a robust affinity Along with the stationary period and moves by slowly and gradually. The elution pace in the column is dependent upon the affinity involving the compound as well